![]() The resulting chimeric embryos showed germline contribution of TPS cells and the presence of TPS-derived trophoblast and visceral endoderm cells. For this, the authors designed and performed chimera formation assays with multiple cells or more stringently, just as a single cell injected into 8-cell embryos. To further substantiate their claims in vivo, the authors examined whether TPS cells have an unbiased ability to contribute to both embryonic and extraembryonic lineage development. Importantly, the cocktail can be successfully employed to derive totipotent-like cells directly from 2-cell mouse embryos without any adaption step. Besides several molecular totipotent features, CPEC-converted cells are also capable of differentiating into extraembryonic cells in vitro. 3 The finding that the CPEC condition as a whole supports totipotency is interesting and novel. Interestingly, RA signaling was recently identified as a critical regulator of totipotency, 4 and DOT1L inhibition was also used in another study as a component for sustaining totipotency in vitro. A condition containing CD1530 (a RARĪ³ agonist), V PA (an HDAC inhibitor), EPZ004777 (a DOT1L inhibitor) and CHIR99021 (a WNT agonist) (collectively, the CPEC condition) turns out to largely and stably induce and maintain cells preserving the totipotent molecular features with intense expression of totipotency markers. 3 performed a chemical screen in extended pluripotent stem (EPS) cells and identified chemicals that can significantly increase 2-cell marker (MERVL-tdTomato/Zscan4-GFP) expression and positive subpopulation. In June issue of Cell Research, Xu et al.
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